Malbac single cell wga kit malbac single cell wga kit rapid reliable and reproducible whole genome ampli cation for single cells nucleic acid analytical platforms e g.
Malbac single cell wga kit.
Malbac single cell wga kit is based on the novel patented malbac technology and provides rapid reliable and reproducible whole genome amplification from single cells or equivalent gdna picogram level to produce 2 4 micrograms of amplified gdna in about 4 hours.
By this re amp kit one can obtain 1 3 µg gdna from 1 10 ng previous malbac amplified products in about 1 hour.
Malbac single cell wga re amp kit is a companion product of malbac single cell wga kit.
The single cell wga kit is intended to be used for research purposes only.
1 the kit includes a robust optimized cell lysis protocol that is incorporated into the.
The re amped products keep good fidelity to the first round malbac products without introducing further amplification bias.
Therefore we recommend at least 100 cells used for analyzing ffpe samples by malbac wga.
Single cell resequencing scrs provides many biomedical advances in variations detection at the single cell level but it currently relies on whole genome amplification wga.
The malbac single cell wga kit performs well on cultured cells white blood cells facs sorted cells fresh or frozen tissues and formaldehyde fixed tissues and cells.
For ffpe samples the amplification efficiency and reproducibility is limited.
Whole genome amplification for single cell s picogram to sub nanogram quantities of gdna based on the patented malbac technology from harvard university malbac s.
Qpcr microarray and high throughput sequencing often require the sample to meet speci c requirements for quantity and quality.
Therefore whole genome ampli cation wga.
Three methods are commonly used for wga.
Multiple displacement amplification mda degenerate oligonucleotide primed pcr dop pcr and multiple annealing and looping based amplification cycles malbac.
The kit is able to achieve consistent amplification efficiency in both at.
Malbac single cell wga kit.
The single cell wga kit may not be transferred to third parties resold modified for resale used to provide commercial services or used to manufacture commercial products without written approval of yikon genomics co.
With 30x sequencing depth 93 of the single cell genome can be covered compared with 50 60 by mda.
Comparison of uniformity of single cell amplification between malbac and mda.
Malbac single cell wga kit provides rapid reliable and reproducible whole genome amplification of single cell s or picogram to sub nanogram quantities of gdna to produce 2 4 micrograms of amplified products in about 4 hours.
